Journal: Life sciences

Article Title: Role of anti-angiogenic factor endostatin in the pathogenesis of experimental ulcerative colitis

doi: 10.1016/j.lfs.2010.10.026

Figure Lengend Snippet: Time-dependent changes in endostatin, VEGF and PDGF protein levels in rat colonic tissue during iodoacetamide-induced UC. Protein levels in colonic tissue were determined by (A) Western blot and (B) ELISA. GAPDH levels were used as loading controls. Assays were repeated 3 times with highly reproducible results using protein from 3 rats/group. **p<0.01; ***p<0.001 vs. control (Ctrl).

Article Snippet: Rat VEGF ELISA kit for cell and tissue lysate (Raybiotech, Inc.; Norcross, GA) and human endostatin DuoSet ELISA Development kit (R&D Systems; Minneapolis, MN) were used according to the manufacturer’s instruction for the measurement of VEGF and endostatin in colonic mucosa.

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: Life sciences

Article Title: Role of anti-angiogenic factor endostatin in the pathogenesis of experimental ulcerative colitis

doi: 10.1016/j.lfs.2010.10.026

Figure Lengend Snippet: The correlation between colonic levels of endostatin, VEGF and size of colonic lesions during iodoacetamide-induced UC. (A) Protein levels in colonic tissue were determined by Western blot. Samples distributed from left to right according to size of colonic lesions from the smallest to greatest. GAPDH levels were used as loading controls. (B) Linear regression report and Spearman’s rank correlation coefficient. The density of Western blot bands was measured by the Eagle Eye II (Stratagene, Austin, TX) and presented in arbitrary units. Assays were repeated 3 times with highly reproducible results using protein from 3 rats/group.

Article Snippet: Rat VEGF ELISA kit for cell and tissue lysate (Raybiotech, Inc.; Norcross, GA) and human endostatin DuoSet ELISA Development kit (R&D Systems; Minneapolis, MN) were used according to the manufacturer’s instruction for the measurement of VEGF and endostatin in colonic mucosa.

Techniques: Western Blot

Journal: Life sciences

Article Title: Role of anti-angiogenic factor endostatin in the pathogenesis of experimental ulcerative colitis

doi: 10.1016/j.lfs.2010.10.026

Figure Lengend Snippet: Changes in endostatin (A), VEGF and PDGF (B) protein levels in colonic tissue from IL-10 KO mice spontaneously developing chronic UC and WT mice on a C57BL/6J background. Protein levels in colonic tissue were determined by Western blot. GAPDH levels were used as loading controls. Assays were repeated 3 times with highly reproducible results using protein from 3 mice/group.

Article Snippet: Rat VEGF ELISA kit for cell and tissue lysate (Raybiotech, Inc.; Norcross, GA) and human endostatin DuoSet ELISA Development kit (R&D Systems; Minneapolis, MN) were used according to the manufacturer’s instruction for the measurement of VEGF and endostatin in colonic mucosa.

Techniques: Western Blot

Journal: Life sciences

Article Title: Role of anti-angiogenic factor endostatin in the pathogenesis of experimental ulcerative colitis

doi: 10.1016/j.lfs.2010.10.026

Figure Lengend Snippet: Levels of endostatin, angiostatin, VEGF and PDGF in colonic tissue of wild-type (WT) and MMP-9 KO mice with DSS-induced UC and water-ingested control. Protein levels in colonic tissue were determined by Western blot. A: Representative Western blot bands of endostatin, VEGF, and PDGF; B: Western blot density of endostatin; C: Western blot density of VEGF; D: Western blot density of PDGF. GAPDH levels were used as loading controls. Assays were repeated three times with highly reproducible results.

Article Snippet: Rat VEGF ELISA kit for cell and tissue lysate (Raybiotech, Inc.; Norcross, GA) and human endostatin DuoSet ELISA Development kit (R&D Systems; Minneapolis, MN) were used according to the manufacturer’s instruction for the measurement of VEGF and endostatin in colonic mucosa.

Techniques: Control, Western Blot